The MOUSE ANTI HUMAN C5 Antibody from MyBioSource.com is a Mouse Monoclonal antibody to C5, C5b, Complement C5, and CPAMD4. This antibody recognizes Human antigen. The MOUSE ANTI HUMAN C5 Antibody has been validated for the following applications: ELISA, Functional Assay, and Western Blot.
Description
MBS212915 recognises secreted complement component 5 (C5), a 190kDa serine protease present in blood serum. It is composed of an 115kDa disulphide linked alpha-chain and a 75 kDa beta-chain. In addition to its role in complement, C5 is thought to be associated with inflammation, stimulating tumour necrosis factor alpha, interferon gamma mediated suppression of IL10 and IL12 production. C5 is activated via either the classical or the alternative complement pathway, by proteolytic cleavage of C5a from the N-terminal of the alpha-chain, by C5 convertase. C5a is an anaphylotoxin which mediates local inflammation. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. It also stimulates chemotaxis of neutrophils, eosinophils, basophils and monocytes towards the site of inflammation. The remaining fragment, C5b, initiates the assembly of the membrane attack complex. In haemolysis inhibition studies it was found that MBS212915 will block C5 haemolytic activity. Removal of sodium azide is recommended prior to use in functional assays. MyBioSource recommend the use of EQU003 for this purpose